冷冻精液输精深度和时间对蒙古羊受胎率的影响

doi:10.12160/j.issn.1672-5190.2021.05.018

畜牧与饲料科学 ›› 2021, Vol. 42 ›› Issue (5): 108-111.doi: 10.12160/j.issn.1672-5190.2021.05.018

冷冻精液输精深度和时间对蒙古羊受胎率的影响

赵霞¹, 马跃军¹, 刘斌¹, 吴铁成¹, 高玉林¹, 李玉荣¹, 乌兰其其格², 苏雅拉图³, 乌仁图亚⁴

1.内蒙古自治区农牧业科学院,内蒙古呼和浩特 010031;
2.锡林郭勒职业学院,内蒙古锡林浩特 026000;
3.赤峰市巴林左旗乌兰达坝苏木动物防疫站,内蒙古巴林左旗 025463;
4.赤峰市巴林左旗畜牧工作站,内蒙古巴林左旗 025450

收稿日期:2020-08-24 修回日期:2021-06-02 出版日期:2021-09-30 发布日期:2021-11-25
通讯作者: 苏雅拉图（1967—）,男,畜牧师,主要从事家畜繁殖技术研究与推广工作。
作者简介:赵霞（1968—）,女,研究员,硕士,主要从事家畜繁殖技术研究与推广工作。
基金资助:
内蒙古农牧业创新基金项目（2020CXJJM05）

Effects of Insemination Depth and Time on Conception Rate of Mongolian Sheep Inseminated with Frozen Semen

ZHAO Xia¹, MA Yue-jun¹, LIU Bin¹, WU Tie-cheng¹, GAO Yu-lin¹, LI Yu-rong¹, Wulanqiqige², Suyalatu³, Wurentuya⁴

1. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;
2. Xilinguol Vocational College,Xilinhot 026000,China;
3. Animal Epidemic Prevention Station of Wulandaba Sumu of Bairin Left Banner of Chifeng City,Bairin Left Banner 025463,China;
4. Animal Husbandry Workstation of Bairin Left Banner of Chifeng City,Bairin Left Banner 025450,China

Received:2020-08-24 Revised:2021-06-02 Online:2021-09-30 Published:2021-11-25

摘要/Abstract

摘要： [目的] 研究冷冻精液输精深度和时间对蒙古羊受胎率的影响。[方法] 选取体况健康、繁殖机能正常的蒙古羊母羊作为试验动物,采用孕酮海绵栓对受体母羊进行同期发情处理。应用常规人工输精法（n=135）、过子宫颈输精法[过子宫颈第一皱褶（1~2 cm）输精（n=120）,过子宫颈第二到第三皱褶（3~4 cm）输精（n=120）]、腹腔镜辅助子宫内输精法（n=128）对发情后的母羊进行冷冻精液人工授精。接受常规人工输精法和过子宫颈输精法处理的受体母羊在发情后约14 h和22 h各输精1次,接受腹腔镜辅助子宫内输精法处理的受体母羊在发情后30~36 h输精1次。输精后40 d用B超仪检测母羊妊娠情况,计算并比较接受不同输精方法处理母羊的受胎率。[结果] 腹腔镜辅助子宫内输精法的受体母羊受胎率平均值最高,为60.2%;其次为过子宫颈第二到第三皱褶（3~4 cm）输精,受胎率平均值为58.3%;过子宫颈第一皱褶（1~2 cm）输精和常规输精法的受体母羊受胎率平均值分别为37.5%和36.3%。统计学分析表明,腹腔镜辅助子宫内输精法和过子宫颈第二到第三皱褶（3~4 cm）输精的受体母羊受胎率平均值显著（P<0.05）高于常规输精法和过子宫颈第一皱褶（1~2 cm）输精。[结论] 蒙古羊母羊发情后30~36 h采用腹腔镜辅助子宫内输精法完全穿刺子宫颈输精1次,可获得比常规输精法更高的受胎率;采用过子宫颈输精法在母羊发情后约14 h和22 h各输精1次,当授精深度接近4 cm时可达到与腹腔镜辅助子宫内输精法接近的受胎率。

关键词: 蒙古羊, 冷冻精液, 过子宫颈输精, 腹腔镜辅助子宫内输精, 受胎率

Abstract: [Objective] To assess the effects of insemination depth and time on conception rate of Mongolian sheep inseminated with frozen semen. [Method] Health Mongolian ewes with normal reproductive capacity were chosen as experimental animals and were subjected to estrus synchronization using progesterone sponges. The ewes were artificially inseminated with frozen semen after estrus using conventional artificial insemination method （n=135）, transcervical insemination method [transcervical first fold （1-2 cm） insemination （n=120）, and transcervical second to third fold （3-4 cm） insemination （n=120）] , and laparoscopically assisted intrauterine insemination method （n=128）, respectively. The recipient ewes receiving conventional artificial insemination and transcervical insemination were separately inseminated once around 14 h and 22 h after estrus, and those receiving laparoscopically assisted intrauterine insemination were inseminated once during 30 h to 36 h after estrus. B-ultrosonic examination was carried out at 40 d after insemination, and the conception rates of the ewes inseminated by different methods were calculated and statistically compared. [Result] The highest average conception rate was observed in the ewes receiving laparoscopically assisted intrauterine insemination （60.2%）, followed by those receiving transcervical second to third fold （3-4 cm） insemination （58.3%）; the average conception rates of the ewes receiving transcervical first fold （1-2 cm） insemination and conventional artificial insemination were 37.5% and 36.3%, respectively. The statistical analysis demonstrated that the average conception rates of the ewes receiving laparoscopically assisted intrauterine insemination and transcervical second to third fold （3-4 cm） insemination were significantly （P<0.05） higher than those of the ewes receiving conventional artificial insemination and transcervical first fold （1-2 cm） insemination. [Conclusion] For Mongolian ewes, laparoscopically assisted intrauterine insemination method （inseminated once during 30 h to 36 h after estrus） that completely penetrates cervix obtains higher conception rate in comparison with conventional artificial insemination method; transcervical insemination method （separately inseminated once around 14 h and 22 h after estrus） with depth of nearing 4 cm achieves similar conception rate to laparoscopically assisted intrauterine insemination method.

Key words: Mongolian sheep, frozen semen, transcervical insemination, laparoscopically assisted intrauterine insemination, conception rate

中图分类号:

S826.34

赵霞, 马跃军, 刘斌, 吴铁成, 高玉林, 李玉荣, 乌兰其其格, 苏雅拉图, 乌仁图亚. 冷冻精液输精深度和时间对蒙古羊受胎率的影响[J]. 畜牧与饲料科学, 2021, 42(5): 108-111.

ZHAO Xia, MA Yue-jun, LIU Bin, WU Tie-cheng, GAO Yu-lin, LI Yu-rong, Wulanqiqige, Suyalatu, Wurentuya. Effects of Insemination Depth and Time on Conception Rate of Mongolian Sheep Inseminated with Frozen Semen[J]. Animal Husbandry and Feed Science, 2021, 42(5): 108-111.

参考文献

[1] NAQVI S M K,PANDEY G K,GUATAM K K,et al. Evaluation of gross anatomical features of cervix of tropical sheep using cervical silicone moulds[J]. Animal Reproduction Science,2005,85(3/4):337-344.
[2] 庞训胜,王锋,尤向峰,等.山羊和绵羊子宫颈管解剖结构的比较[J].畜牧与兽医,2006,38(6):46-47.
[3] MAXWELL W M C,HEWITT L J. A comparison of vaginal,cervical and intrauterine insemination of sheep[J].The Journal of Agricultural Science,1986,106(1):191-193.
[4] SAYRE B L,LEWIS G S.Fertility and ovum fertilization rate after laparoscopic or transcervical intrauterine artificial insemination of oxytocin-treated ewes[J].Theriogenology,1997,48(2):267-275.
[5] EPPLESTON J,MAXWELL W M C. Recent attempts to improve the fertility of frozen ram semen inseminated into the cervix[J].Wool Technology and Sheep Breeding,1993,41(3):291-302.
[6] 李宇,王敏,宋博宇,等.山羊精液冷冻保存研究进展[J].家畜生态学报,2019,40(7):82-85.
[7] HALBERT G W,DOBSON H,WALTON J S,et al.The structure of the cervical canal of the ewes[J].Theriogenology,1990,33(5):977-992.
[8] ANEL L,ALVAREZ M,MARTINEZ-PASTOR F,et al.Improvement strategies in ovine artificial insemination[J].Reproduction in Domestic Animals,2006,41(Suppl 2):30-42.
[9] EPPLESTON J,SALAMON S,MOORE N W,et al.The depth of cervical insemination and site of intrauterine insemination and their relationship to the fertility of frozen-thawed ram semen[J].Animal Reproduction Science,1994,36(3/4):211-225.
[10] ARMSTRONG D T,EVANS G.Intrauterine insemination enhances fertility of frozen semen in superovulated ewes[J].Journal of Reproduction and Fertility,1984,71(1):89-94.
[11] KAABI M,LVAREZ M,ANEL E,et al.Influence of breed and age on morphometry and depth of inseminating catheter penetration in the ewe cervix:a postmortem study[J].Theriogenology,2006,66(8):1876-1883.

[1]	李长青, 王利, 金海, 薛树媛, 郭天龙, 王超, 张海鹰, 田丰. 戈壁草原蒙古羊春季羔羊铜缺乏症的研究[J]. 畜牧与饲料科学, 2022, 43(2): 46-50.
[2]	贾明蕊, 何江峰, 王力伟, 陈欣, 方勤圆, 张林, 董鹏, 刘永斌. 蒙古羊的起源及尾脂沉积的研究进展[J]. 畜牧与饲料科学, 2021, 42(3): 70-73.
[3]	乌日勒格, 李九月, 田丰, 王利, 高源, 斯钦孟和, 薛树媛. 围产期补饲对放牧蒙古羊母羊泌乳性能及羔羊生长性能的影响[J]. 畜牧与饲料科学, 2020, 41(5): 27-32.
[4]	王德宝, 郭天龙, 王莉梅, 张园园, 李星云, 纳钦, 康连和, 杨燕燕, 翟琇, 梁俊芳. 不同屠宰方式对宰后蒙古羊羊肉AMPK活性、糖酵解及羊肉品质的影响[J]. 畜牧与饲料科学, 2020, 41(3): 47-50.
[5]	何小龙, 刘学文, 常福恩, 李思勤, 付绍印, 达赖, 王标, 祁云霞, 特日格勒, 刘永斌. 不同品种受体对绵羊胚胎移植效果及羔羊早期生长性能的影响[J]. 畜牧与饲料科学, 2019, 40(6): 20-23.
[6]	胡文举, 宋艳画. 谷胱甘肽对犬精液冷冻保存效果的影响[J]. 畜牧与饲料科学, 2019, 40(11): 65-69.
[7]	达赖,付绍印,刘斌,何小龙,王标,陈欣,方勤圆,张兴夫,姜卓,张文广,刘永斌. 蒙古羊不同尾椎数相关选择信号的挖掘研究[J]. 畜牧与饲料科学, 2019, 40(1): 28-33.
[8]	张永昌,娜仁花,达拉吉如嘎,刘永斌,吴海青. 非繁殖季节母驴诱导发情、排卵效果的研究[J]. 畜牧与饲料科学, 2018, 39(9): 10-18.
[9]	何小龙,金海,刘永斌,荣威恒. 草原戈壁短尾羊的起源及选育进展[J]. 畜牧与饲料科学, 2018, 39(7): 49-49.
[10]	张博宇,任俊光,武迪,刘佳,李勇,刘泽成,白恩宇,王宇航,郑容秀,杨燕燕. 绵羊输卵管移植和子宫角腹腔镜微创移植2种胚胎移植方法受胎率的比较分析[J]. 畜牧与饲料科学, 2017, 38(7): 42-42.
[11]	杨燕燕,杜文,刘永斌,达来,王标,青格勒,周璇,何亭漪,乌达巴拉,田瑛. BMP6基因在单、双羔蒙古羊不同生理时期卵巢组织中的差异表达及分析[J]. 畜牧与饲料科学, 2017, 38(3): 25-25.
[12]	包牧仁,李强,孙洪岩,娜仁图雅,戴雪. 绒山羊冷冻精液配种技术的应用效果[J]. 畜牧与饲料科学, 2017, 38(10): 53-53.
[13]	田瑛,杜文,达来,周旋,杨燕燕,乌达巴拉,青格勒,杨峻明,武金栋,刘贵忠,李靖. 杂交对杜泊羊与蒙古羊杂交后代屠宰性能及肉品质影响[J]. 畜牧与饲料科学, 2016, 37(10): 28-28.
[14]	杨秀清,玉梅,乌仁其其格. 应用人工授精技术提高母牛受胎率[J]. 畜牧与饲料科学, 2015, 36(2): 108-108.
[15]	索朗达,布次仁,白玛罗布,强巴索朗,巴桑参绝,宗吉,边普,益西多吉. 提高藏西北绒山羊受胎率的主要措施[J]. 畜牧与饲料科学, 2015, 36(12): 87-87.

冷冻精液输精深度和时间对蒙古羊受胎率的影响

Effects of Insemination Depth and Time on Conception Rate of Mongolian Sheep Inseminated with Frozen Semen

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价